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An alternative, but not necessarily independent explanation is that substitution of Q169 altered hTERT conformation, which prevented efficient nucleotide incorporation (below).
On the other hand isoproterenol, a cAMP raising agonist, diminished the rate of thymidine incorporation below the basal rate of serum-deprived cells.
Although in most cases UV-irradiation may be sufficiently effective on its own to reduce H-thymidine incorporation below that of 5% of the non-irradiated control, the combination of gamma-irradiation and UV-irradiation adds an extra layer of security in minimizing the possibility of tumor outgrowth following vaccination.
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Then either 3- 4,5-dimethyl-2-thiazolyl -2,5-diphenyl-2H-tetrazoliumbromide (MTT) reduction (cf below) to quantify the number of metabolically active viable cells, thymidine incorporation (cf below) to quantify DNA synthesis or measurement of nucleosome fragments to quantify apoptosis, were performed (cf below).
Freestanding SCD plates showed high crystalline quality without birefringence and had nitrogen incorporation levels below 160 ppb.
The resulting solution was incubated at 62 °C for 15 min. Extent of editing was evaluated using AMV-RT-catalyzed ddNTP incorporation (see below).
One thousand, one hundred sixty-two proteins had an incorporation rate below 20%, which we hypothesize are housekeeping proteins that largely pre-exist in the macrogametocyte and turn over slowly in the differentiating ookinete.
However, as will be further discussed below, the incorporation of active molecules as a coating has several constraints and only can be made when the proportion of these compounds in fertilizer formula is low (normally below 1%%).
The incorporation of titanium below 12.3 at.% dropped the room temperature wear rate significantly.
Notably, at higher ligand concentrations, the observed anti-proliferative response was even more pronounced than when the cells were grown under serum-free conditions, as the levels of BrdU incorporation dropped well below basal levels.
See the "Web Incorporation Service" section below for further instructions.
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