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Classic bromodeoxyuridine (BrdU) incorporation analysis, phospho-histone3 immunostaining and hydroxyurea (HU) (S-phase inhibitor) treatment showed that FEVPs are proliferating at the LPM.
The cell supernatant and insoluble pellets then were further treated for HPLC assay and DNA incorporation analysis with the procedures described above.
Consistent with what was observed in 3H incorporation analysis, antigen stimulation induced faster proliferation rates than CD3 mAb stimulation (Fig. 4A).
Together with our NA incorporation analysis, the NA activity is determined by the character of each NA, particular the ectodomain not the transmembrane domain.
Although unable to interact with FlnA, the mutant AR still mediates androgen-induced DNA synthesis as shown by BrdU incorporation analysis of AR-negative human mammary cancer-derived MDA-MB231 cells ectopically expressing this mutant (Fig. 9E).
To see if there is any abrogation of p53 function to induce growth arrest in the EJ-53 cells, thymidine incorporation analysis indicates that inhibition of BAG-2 by RNA interference (RNAi) increases thymidine incorporation as an index of cell proliferation (Fig. 5C).
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Similarly, Raidd deficiency had no impact on homeostatic or c-Myc-driven B-cell proliferation, as assessed by BrdU-incorporation analysis in immature CD19+IgM− and CD19+IgM+ B cells from bone marrow or spleen of premalignant mice.
In order to find out the extent of strain that have been induced in the lattice due to cobalt incorporation, strain analysis has also been carried out.
This suggests that incorporation of analysis of TMD sequences could improve the accuracy of current methods for predicting subcellular localization.
As well as phospholipid incorporation, TLC analysis revealed a significant increase in fatty acid accumulation in non-polar lipids (Fig. 3C,D), which correlates with the observed lipid droplet accumulation in Dictyostelium and hepatocytes.
In contrast to single-nucleotide incorporation assays, analysis of full-length extension products in the presence of all four dNTPs can provide a measure of truncated, untargeted (i.e., random), mutations and/or frameshifted extension products.
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