Exact(1)
Incorporating the efficiency difference between PminC and PminL, the Ct difference after adjustment (ΔCt' = 4.38) was slightly larger than that without adjustment (ΔCt = 4.00).
Similar(59)
The expression levels of the samples were calculated using the ΔCt method, incorporating the efficiencies of each primer pair.
From the results of Table 4, it is clear that when we incorporate the efficiency of the processes in calculating the total efficiency, the same results are obtained.
Later, Manoranjan and Lee [17] studied Fishers equation, which is the logistic growth model which they modified to arrive at a population model that incorporates the efficiency of resource utilization based on the idea of adsorption theory in chemical kinetics.
A breeding program can then use this information to incorporate the efficiency constituent traits into an index to achieve genetic progress.
Primers were validated and the results analysed as described in [ 82] using the methods of [ 105, 106], which incorporates the efficiency of the primers as a factor in the equation.
The relative expression level and FC of each target gene in R and C relative to S were calculated according to the 2-ΔΔCT method incorporating the PCR efficiency [ 27] after normalization with the housekeeping genes ribosomal protein S7 (RSP7; andP010592) actinctin 5C (AGAP00065C).
The relative expression ratio was calculated for set 1 using the delta-delta-Ct method and for set 2 applying a mathematical model incorporating the PCR efficiencies and the crossing point deviation of EGF-treated HeLa cells- versus control non treated cells at each time point.
To measure its unified efficiency, we need to incorporate the two efficiency frontiers into the proposed analytical structure, as visually described in Fig. 1.
The effective CO2 storage capacity in the H-59 block is estimated to be 26.37 × 104 ton by incorporating the CO2 sweep efficiency and neglecting the mineral trapping.
Expression levels were calculated using the CFX manager 2.1 software (Biorad) via the delta-Cq method, incorporating the calculated amplification efficiency for each primers pair.
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