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Genomic DNA was extracted from the parents and the 94 seedlings following a scaled-down CTAB extraction [ 28] incorporating the addition of 1% [v/v] β-mercaptoethanol and 2% [w/v] polyvinyl pyrollidone (PVP 40) to the extraction buffer.
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Others have proposed that the framework is sufficiently flexible to incorporate the addition of wellness themes or concepts [ 46].
Plans to modify the existing MRC sponsored ICON8 study to incorporate the addition of bevacizumab yet retain a third arm of dose dense therapy without bevacizumab are most attractive, but execution of these may be challenged by austerity measures.
As the EORTC 22921 and FFCD 9203 trials used 5FU LV-based CRT schedules, it was a logical step to develop preoperative radiation schedules that incorporate the addition of oxaliplatin to this regimen.
As the EORTC 22921 and FFCD 9203 trials used daily 5FU LV based CRT schedules, at the time the present study was designed, it was a logical step to develop preoperative radiation schedules that incorporated the addition of irinotecan to the same regimen.
When this type of chaplet was adopted by the nobility in general, the royal crown was distinguished by a number of ornaments upstanding from its rim; by the 15th century the helmet form was incorporated by the addition of one or more arches.
In order to avoid such a simplification, we incorporated the additional operations of addition and subtraction in the definition of the message transformation functions as shown above.
If they can be incorporated into clothing, the addition of other nano-sized devices could allow a fully wearable communications centre to be made.
A post-operative intervention incorporating these elements with the addition of PERT may be effective in resolving residual impairments after surgery.
In addition, incorporating the effects of environmental variation [82], multiple human stressors [83], and linkages in interaction webs [84], [85] with socioeconomic factors that lead to overfishing [86] may improve management and conservation in coral reef systems.
These transport reactions were incorporated into iRsp1095 by the addition of 15 genes, 2 reactions and one metabolite (Additional file 3: Tables S4).
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