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The National Cancer Institute Precision Medicine Initiative is an unprecedented large interdisciplinary collaborative effort to conceptualize and test the feasibility of trials incorporating sequencing platforms and large-scale bioinformatics processing that are not currently uniformly available to patients.
To avoid incorporating sequencing errors into the data, several expressed sequence tags (ESTs) with similar sequences were aligned and amplification primers were designed to highly conserved regions.
Members of clusters represent alleles, recently derived paralogs, or contigs incorporating sequencing errors with differences below the resolution afforded by our sequencing read lengths.
We generate simulated RNA-seq data using simNGS [ 16 ] (http://www.ebi.ac.uk/goldman-srv/simNGS/ ), incorporating sequencing errors, and setting all transcripts to be equally expressed.
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Replication timing was determined as the ratio of early-to-late BrdU incorporating sequences.
By incorporating sequences encoding amiRNAs specific to 53R of RGV into pre-miRNA155 (pSM155) vectors, which use the backbone of natural miR-155 sequence and could intracellularly express 53R-targeted pre-amiRNAs.
Overall, all methods can improve significantly their accuracy by incorporating sequence information.
A global fit to the minimal kinetic scheme incorporating sequence dependence (solid lines, Figure 6A I) suggests 'partially' active unwinding.
By incorporating sequences from multiple coccidian species into HMM profiles, we were able to identify several putative ROPKs that were not identified in previous computational surveys [ 9, 10].
Our aim is to compare and evaluate the phylogenetic trees based on COI with independent evidence from the nuclear ITS2 incorporating sequence, as well as, secondary structure information.
> In Figure 2, we consider the effect of sequentially incorporating sequence-based features according to the decreasing order of their kernel performances.
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