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In this work, high-temperature stable 2-μm nanostructured surfaces were prepared by ultraviolet-based nanoimprint lithography using an alkoxysilane binder incorporating modified silica nanoparticles.
The effect of incorporating modified clay into a polyamide 6 (PA6) matrix, on the free volume cavity sizes and the thermal and viscoelastic properties of the resulting nanocomposite, was studied with positron annihilation lifetime spectroscopy, differential scanning calorimetry and dynamic mechanical analysis.
In contrast, labeling by incorporating modified nucleotides or label into an extended poly A tail, or by labeling with a modified primer by first strand cDNA synthesis, should produce a more even label; 3) We found that amplification techniques such as RT-PCR or T7 promoter expression, produce different expression patterns on arrays when compared to un-amplified samples.
Following completion of the study the authors became aware of a GP5+/GP6+ protocol incorporating modified ramping times (MRT) [ 13].
Assays can be improved by incorporating modified bases or by introducing a secondary mismatch within the primer [ 21- 24].
Linearly amplified aRNA was generated from 0.5 μg totRNA using the MessageAmp aRNA kit (Ambion) according to the manufacturer's instructions and incorporating modified 5- 3-aminoallyl -UTP.
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Non-natural amino acid mutagenesis has enabled us to incorporate modified amino acids into specific positions of proteins in both cell-free and in-cell translation systems using tRNAs that are aminoacylated with modified amino acids.
During the experiment, categories or levels were incorporated, modified or excluded from the original scale.
PEPstrMOD implements the original PEPstr algorithm and incorporates modified residues using force field libraries.
A main advantage of peptide arrays is the possibility to incorporate modified and non-natural amino acids.
This method may have applications for evolving other polymerase variants with novel phenotypes, such as the ability to incorporate modified nucleotides.
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