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The specificity of the MassCode system was enhanced by incorporating a probe interrogation step.
Of particular interest was validating multiplex PCR results by incorporating a probe extension step.
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We identified the pipelines that were suitable to reduce the technical variability and highlighted that only those incorporating a probe-type correction step provided consistent measurements for DM analysis.
Taken together, the observed reactivity of HRP@HSNs to ROS, evaluated by incorporating a fluorescent probe (DHR123), could be resulted from a combination of the affinity of the nanoparticles as well as intrinsic property of HRP for ROS.
A novel electrogenerated chemiluminescence (ECL) aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe was designed by employing specific anti-thrombin aptamer as a capture probe and a ruthenium II) complex-tagged reporter probe as an ECL probe and an auxiliary probe to assist the ECL probe close to the surface of the electrode.
Copy number can be evaluated in tumors and the two different causal genetic mechanisms can be distinguished through the use of dual-color FISH analysis incorporating a gene-specific probe combined with a centromere-specific probe for the chromosome encoding that gene.
An unshielded gradient set (Varian Associates) was used with a home-built probe incorporating a two-turn surface coil probe (20 mm diameter) tunable to frequencies of 400 MHz (H-imaging) and 162 MHz (P-spectroscopy), although only proton imaging was performed in this study.
An electrogenerated chemiluminescence (ECL) biosensor array for the individual and simultaneous detection of multiple biomarkers has been developed at first time on the basis of the aptamer-based biosensor incorporating a versatile signal probe.
By building a retractable probe incorporating a versatile target holder, and modifying the ion optics of a commercial mass spectrometer, we show that we can steer the macromolecular ion beam onto a target for imaging by means of transmission electron microscopy and atomic force microscopy.
Our next goal was to develop an imaging probe incorporating a facile and rapid radiochemical synthesis along with biological traits similar 11C-MBZA.
Greater analyte specificity should be possible by incorporating a fluorescent molecular beacon probe specific to an internal region of the target amplicon, thus minimizing non-specific signal.
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