Sentence examples for incorporated upstream from inspiring English sources

A third marker, fluorescent protein mKusabira orange mKO) flanked by loxN pair (loxN-mKO-pA-loxN) was incorporated upstream of the STARS cassette to facilitate selection of cell lines that have the construct integrated in the genome, and to prevent cells from expressing M-GFP in the absence of Cre (Fig. 2a).

Three additional copies of the cMyc epitope were incorporated upstream of mCherry using primers KS3709 and KS3710.

Additional linker sequence, 5'-GGCGGCTCCGGCGGCGGCTCCGGC-3' which corresponds to GGSGGGSG amino acid linker sequence was incorporated upstream to the cA sequence for gene synthesis.

The enhancer UAS regulatory region was incorporated upstream of all constructs (UAS-minigene:Luc), thus allowing the use of the UAS-Gal4 system, commonly used in Drosophila for the accurate control of transgene expression (Brand and Perrimon, 1993).

Tiling also incorporated 2500 bases upstream and 500 bases downstream of each UCR.

During the growth of sand patches, sand particles transported from upstream are incorporated into sand patches and decelerated (like cars on a congested road); this local state is not in equilibrium, but in a stochastic (statistical) sense, it does not violate the equilibrium of the entire system, because sand patches repeatedly appear and disappear in many places.

Primers JK74 and JK75 incorporated an AvaI site (CCCGAG) upstream of the start of the CTD sequence.

It did so by emphasizing a three-pronged approach that incorporated research and action for understanding the upstream causes of ill health and inequity and for taking action on these causes, toward solving pressing LMIC health challenges with global significance.

The PrRP-Cre targeting vector contained an IRES-Cre transgene inserted just downstream of the endogenous STOP codon in PrRP exon 2. The LSL- PrRP targeting construct incorporated loxP sites flanking a transcription STOP cassette just upstream the endogenous PrRP gene and a neomycin-resistance cassette.

To clone the patA promoter into pBAV1K2, giving pBAV1K2 p, a 144-bp DNA fragment that covered the region from 12 to 146 bp upstream of the patA start codon and incorporated a 5′ EcoRI site and a 3′ XbaI site was synthesized by GeneArt (Life Technologies).

The long splicing isoform contained a cryptic exon of 317nt resulted from the use of a preexisting 3′ splice site that locates 108 nt upstream of Ank1 IVS13+209T>C mutation; the short splicing isoform incorporated two cryptic exons defined by using aforementioned 3′ splice site and two other preexisting 5′ and 3′ splice sites at downstream of Ank1 IVS13+209T>C mutation.