These dyes were incubated for 30 min, the cells were detached with a sterile cell scraper (Corning Incorporated), transferred to FACS tubes and analyzed by FACS as soon as possible using 488 nm excitation with fluorescence emission at 530 nm (e.g., FL1) and >575 nm (e.g., FL3) (Calibur, Becton Dickinson Immunocytometry Systems, Becton Dickinson, Franklin Lakes, NJ, USA).
When it is well incorporated, transfer to a container with a tight-fitting lid, and refrigerate at least 4 hours, preferably overnight.
Carbonaceous transfer layers were formed on the AZ91 surfaces and incorporated F transferred from F-H-DLC.
Each test incorporated non-transfer and transfer items, the difference of which was that the latter were more difficult than the former.
As described previously (Bundock and Hooykaas [1996]), the T-DNA can either be integrated into the host genome or transferred and incorporated as stable, extra-chromosomal T-DNA elements that can form circular structures in plants (Singer et al. [2012]).
Major foreign banks are trying to enlarge their branch network in China, because new regulations stipulate that once the banks are incorporated, existing branches can be transferred to the new entity, which can engage in renminbi business.
In the group A, CLA was added to the diet of the dams during suckling period and therefore CLA was incorporated in breast milk and transferred to the suckling pups.
Thirteen weeks later, the bone flaps with rhBMP-2-incorporated DFDBA or CHA were transferred to repair segmental mandibular defects.
Immunohistochemical and mRNA expression studies in C. magister, demonstrated that cryptocyanin is synthesised in the hepatopancreas, secreted into the hemolymph, transferred across the epidermis and incorporated into the extracellular matrix of the new exoskeleton [ 14].
Captured monomers either dissociate (at rate constant koff= kon × KD) or are transferred to the tip and irreversibly incorporated with rate constant kt.
Now ownership has been transferred to Whyte via Wavetower, a newly-incorporated company formed for the purpose of acquiring the club from Murray International Holdings and which is 100% owned by Liberty Capital - itself ultimately owned by the London-based businessman.
