Given the distinct differences in expression patterns of ΔN and TA, we incorporated transcript-specific data information for p63 isoforms in our analysis of the expression dataset.
3Omics is specifically designed for the analysis of human data because all of the incorporated transcripts, proteins, metabolites, pathways, and gene information are human-specific.
Finally, we constructed the LINE FUSION GENES database for chimeric transcripts containing L1-5'UTR heads and cellular sequence tails (102) and L1-3'UTR incorporated within transcripts tails (676), and the LINE elements that led to novel splice variants (632).
An increasing ladder of transcripts of the expected sizes from minigene MGBR2i_ex19 24 to 19 27 could be observed, as exons 25, 26, and 27 were successively incorporated in the transcript, respectively.
Thus, the sense transcription of an FL-L1 element and/or the transcripts, incorporated in cis into the chromatin, are essential for the formation and function of a neocentromere and the selective repression of genes within or adjacent to this domain [ 67].
FU incorporated into nascent transcripts was detected using anti-BrdU antibody as described above.
pFL-BRCA1in is identical to pFL-BRCA1 except that two downstream introns were incorporated to render the transcript sensitive to EJC-NMD in addition to miRNA.
Any additional themes that arose from the transcripts were incorporated (see table 2 for a complete list of coding categories).
All teleost long form transcripts were incorporated, as the catalytic domain phylogeny (Additional File 3) suggested the presence of two teleost PDE4A, PDE4B, and PDE4C clades.
After review of these junctions in the context of all available data, another 8841 were incorporated into new transcripts (254 previously incorporated exon junctions were withdrawn).
Because the RNA capture set incorporated 19% of the novel transcript features detected by RNA-Seq, we believe these data can be more reliably extrapolated to the whole RNA-Seq dataset.
