Your English writing platform
Free sign upSuggestions(5)
Exact(2)
An additional step was incorporated to remove genomic DNA using a RNAse-free DNAse kit (Qiagen).
Upon verification of successful amplification by gel analysis, each plate of second round PCR products was purified using Multiscreen-PCR plates from Millipore according to the manufacturers specifications, except that a pre-wash was incorporated to remove potential surfactant type agents present in at least some batches of the filtration units.
Similar(56)
To reduce the FP rate, MAA incorporated several filters to remove "incorrect" annotations caused by nonspecific, partial, and low relevance chemical names.
The membrane with the highest percentage of Ag incorporated was able to remove close to 7-logs of E. coli when operated in the PMR.
This oligo was radiolabelled by filling in the 3′ recessed end of the complementary 16-bp strand with [ α-P] dTTP, followed by purification with QIAquick nucleotide removal kit (Qiagen GmbH, Hilden, Germany) to remove the incorporated radioactive probe.
By using EDTA to remove the incorporated Cu2+, the polymeric film can repeatedly detect Cu2+ with good reversibility and film stability, as supported by XPS and TEM analyses.
After treating the RH with 10% HCl and 30 wt.% sulfuric acid solution, the material was burned in a muffle furnace at 600°C for 4 h to remove all incorporated hydrocarbons.
cDNA were further purified by utilizing CentriCept gelfitration plates to remove non incorporated primer and dNTP.
Subsequently, parasites were harvested and washed twice with ice-cold TBS (10 mM Tris/HCl, pH 7.4, 144 mM NaCl) to remove non-incorporated label.
IRNA transcript was generated from the linearized pIRNA plamsmid using T7 Megascript transcription kit and was purified using G-25 spin columns to remove un-incorporated nucleotides and enzymes.
The RNA was purified from a 15% polyacrylamide/urea gel as described above to remove non-incorporated adapters.
More suggestions(1)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com