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The two previous incorporated steps enabled the gradients to be run in a common laboratory centrifuge instead of an ultracentrifuge (2,600×g, 14.5 hours, swinging bucket rotor/or 36,500×g, 1 hour, Sorvall SH 80 rotor).
During this process, we incorporated steps specifically designed to sequence transcripts from diverse genotypes, tissues, and environmental conditions; identify highly-likely SNP positions; and maximize the number of SNPs that can be reliably assayed using an Illumina Infinium II SNP array.
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The development process described here incorporated three steps to improve validity.
The device incorporated all steps of single-cell processing, including cell capture, cell lysis, reverse transcription, and quantitative PCR.
Parker's hypothesis incorporated incremental steps in the origin of complex system, with circuits arising from sensory neurons/receptors, motoneurons/effectors and interneurons within the nervous system itself.
All Fe products were incorporated in step II (GP) (unpublished results).
To remove any correlation between gene-set size and AUC values, we incorporated a step into our algorithm that repeats cross-validation for randomly selected gene sets.
Shakira incorporated belly dancing steps into her choreography during the performance.
The song, which took four days to rehearse, saw Chopra execute a contemporary mujra, that incorporated complicated dance steps.
For Cuffdiff, the commonly used fragments per kilobase of transcript per million mapped fragments (FPKM) value [ 29] in pair-end sequencing experiments incorporated two normalization steps; i.e., the number of fragments was normalized by the transcript's length and the total yield of the fragments to ensure accurate quantification of the gene's expression [ 27].
Bicycle ride length was also recorded on a daily basis and incorporated into the step-count by the GCC® (6.4 km = 10,000 steps).
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