We genetically removed the intein by sequentially cloning two fragments (amplified from P. furiosus genomic DNA, ATCC) incorporating a silent NotI mutation at the junction.
Cinema appreciation in Ireland can be traced back to the final years of the 19th Century, when eager Dubliners would gather in the city centre to watch silent films incorporated into variety shows.
Around TSSs, H3.5 was incorporated into both active and silent genes, while H3.3 was predominantly incorporated into active genes (Fig. 8d).
The film, directed by Christophe Gans, was based largely but loosely on the game, incorporating elements from Silent Hill 2, 3, and 4. Gans replaced Harry Mason with a female main protagonist, Rose Da Silva, because he felt Harry had many qualities typically perceived as feminine.
Custom synthesized oligonucleotide primers with the desired mismatches were used to incorporate the required silent mutation, preferably incorporating alongside, a restriction enzyme recognition site for future clone confirmation.
During the construction of the full length NF1 expression construct, in the same vector backbone including an N-terminal GFP, silent mutations were incorporated at positions 409 and 813 of the D. discoideum axeB cDNA sequence to introduce a XhoI and a XmaI site, respectively.
In addition a PstI silent restriction site was incorporated into the mutant primers for candidate screening.
A silent restriction site was incorporated in each to assist rapid identification.
A silent restriction site was incorporated into each primer to assist rapid identification.
Cool, silent, aloof, this Freud is wholly incorporated into a medical training system that thinks in terms of disease models.
