This study, which incorporated repeat measurements during pregnancy, allowed detailed temporal analysis of the long-term associations of GWG with several cardiovascular disease risk factors.
We designed an "overlap" method that incorporated repeat masking data generated using the RepeatMasker platform [ 45] to identify genomic positions of the same 7080 annotated Hexapoda TEs sequences from RepBase [ 42].
We incorporated repeats of the ideal coiled coil up to 29 residues long at either end or both ends of our construct, truncating a few residues from either the N- or C-terminal end to match the register of the predicted heptad repeats for the NEMO sequence.
Only two cohorts incorporated repeat dietary measures (Cust et al, 2007a; Larsson et al, 2007), one of which used a single 24-h recall in a stratified random sample of participants in addition to an FFQ (Cust et al, 2007a), whereas the other obtained dietary information using an FFQ at two different time points (Larsson et al, 2007).
The FFQs used were quite variable in length, ranging from 61 items to 192 items in length and only two studies incorporated repeat dietary assessments at different time points to account for potential changes in dietary habits during the follow-up period (Cho et al, 2003; Holmes et al, 2004).
In [13, 14], the cyclostationarity of an OFDM signal is incorporated by repeating symbols in frequency and time, respectively, for the purpose of user network identification.
We incorporated this idea and repeated the hybridization step after the 2nd PCR.
To validate that the observed increase in cell-associated fluorescence was indeed generated by the metabolically incorporated alkyne sugar, we repeated the same experiments using the Lec2 Chinese hamster ovary (CHO) cell mutant.
For this analysis, plots were incorporated as random effect since repeated measurements were used in this study, thus using LMM or GLMM for this analysis.
We also tested a design strategy that incorporated multiple repeats of the MPER epitopes within gag, which significantly increased specific antibody titers, systemically and mucosally.
