Sentence examples similar to incorporated mutants from inspiring English sources
Chemical modification at the single cysteine in the Cys-incorporated mutants was performed by reaction with the thiol-specific probe 5- {2-[(iodoacetyl)amino]ethyl}amino)naphthalene-1-sulfonic acid (IAEDANS) (Molecular Probes) in 100 mM Tris-HCl buffer at pH 8.5- {2-[ reduciodoacetylssible intermoleculamino]ethyl}aminos of the cysteinaphthalene-1-sulfonics with 10 mM DTT.
Strikingly, to date, Uaas incorporated by mutant RSs derived from PylRS bear close chemical and steric resemblance to the natural Pyl substrate.
Upon photoactivation, these mutants incorporated ∼1 mole of the reagent per mole of rhodopsin over time after illuminating the samples for 30 sec.
Extended primers were separated by capillary electrophoresis in the Genetic Analyser 3500 Dx (Applied Biosystems), and the presence or absence of a mutation was indicated by the incorporated wildtype or mutant labelled dideoxy nucleotide.
To date, nearly all Uaas incorporated by evolved PylRS mutants contain a carbonyl group at the Nε position of Pyl.
We compared the combinations for the incorporated enzymes between the mutants showing underestimated and overestimated model errors, clarifying that the members of the incorporated enzymes and the reaction 7 (gltA) are critical.
RbΔf mutant myotubes incorporated BrdU (Fig. 4E, G).
The basal mutant (C140A/167A/185A/222A/264A/316A) incorporated ∼0.5 mole of the reagent per mole of rhodopsin in the dark or after illumination for 30 sec.
This increases the precision to 93% on one-third of the database if FOLD-X analysis is incorporated along with I-Mutant [ 73].
WNV VLPs that incorporated a fusion-loop mutant (W101A) did not dequench upon pH drop and instead released and washed away.
Rpl11-mutant fish incorporated 2.5-fold more deoxycytidine into DNA than their wild-type siblings (Fig. 6A), whereas embryos that had been injected with a morpholino against Rps19 incorporated sevenfold more deoxycytidine in comparison with controls (Fig. 6B).
