Next, we manually incorporated information about host pathways that are activated in response to influenza virus infection, and - for all validated interaction partners of IAV factors - we included information about downstream signalling and processing events (e.g., phosphorylation cascades).
Then, straw from the whole plot was harvested, weighed in the field, and either manually incorporated (into the topsoil) or removed according to treatments.
Seven days before sowing of the first crop (wheat var condor), sewage sludge and humentos were manually incorporated (1 week before each crop in the rotation) in the top 30-cm soil depth.
Finally, the NaOH pellets were incorporated, and the geopolymer mortar was mixed manually for an additional 10 min.
We also incorporated experimentally supported TF miRNA regulatory relations that were curated manually from large numbers of published papers [ 25] and from TransmiR [ 21].
It is well known that in clinical daily practice K-wires are often drilled without proper cooling because most drilling devices do not have an incorporated cooling system and therefore cooling has to be done manually.
However, the links between the incorporated ontology concepts, models and model-related data need to be defined manually.
To this end, we designed a sample holder that incorporated a dual axis translation mount (LM1XY, Thorlabs) and allowed the user to manually move the sample in two directions and center it on the rotation axis (supplementary material Fig. S7).
Data from porcine samples were extracted and analyzed with manually set gates (Kaluza, Beckman Coulter, Brea, CA, USA), since the incorporated pre-set gates of the hematological analyzer were not suitable for porcine circulating cells.
Each SPECT scan was reconstructed iteratively (10 subsets, 12 iterations, and two-pixel-wide [9.6 mm FWHM] 3-D gaussian post-filtering), employing an algorithm that incorporated attenuation correction based on the CT scan derived from the PET/CT study that was manually registered to the SPECT, as well as compensation for the system resolution of the gamma camera.
