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Also formation and drying of films applied from the resulting labelled dispersion induces a significant wavelength shift of the fluorescence of the incorporated label.
Analysis of incorporated label into 4 defined sections of the electrophoretic pattern illustrated quantitative differences.
Analysis of the incorporated label into defined sections of the electrophoretic patterns revealed small quantitative differences between primary and secondary cells.
In all our experiments, the incorporated label in denaturing polyacrylamide gels coincided with the bipartite tRNA detected by ethidium bromide staining.
In the CILAIR experiment we identified 192 proteins with incorporated label in medium of LPS treated tissue and 209 in medium of untreated adipose tissue.
The degree of labeling, which is the ratio of incorporated label to the total concentration of monomeric peptide, was between 3% and 6% for both dyes.
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Fig 3B shows that both plasmids incorporated labelled nucleotide, and in both cases, this incorporation was DDK-dependent.
One limitation of TdT, an enzyme widely used for end labeling of DNA oligomers, is that it is difficult to control the number of incorporated labels, because it shows template-independent extension with random nucleotides.
To this end, we incorporated labelling of surface-exposed RTX on RAW cells with anti-human antibodies (recognizing the Fc portion of this chimeric antibody).
When UV-treated nuclei were incubated in metaphase cytoplasm, they incorporated labeled dUTP, a marker of DNA repair [28], and the number of H1 foci increased approximately 3-fold, however they did not completely overlap with the sites of DNA repair, suggesting a role in some but not all damaged loci (Figure 3C).
Subsequently, parasites were harvested and washed twice with ice-cold TBS (10 mM Tris/HCl, pH 7.4, 144 mM NaCl) to remove non-incorporated label.
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