The synthetic nucleotide sequence, named DP, was incorporated into the expression vector pCMV6-XL5 using a standard DNA recombination procedure, resulting in the recombinant plasmid pCMV-DP.
With human FKRP constructs, two different amino acid substitutions, the same as mutations found MDC1C (1364C > A, A455D) and LGMD2I (826C > A, L276I) patients, were incorporated into the expression vector by site-directed mutagenesis kit (Stratagene).
In order to account for the exquisite pH dependence of the steady-state concentration of 2,3-bisphosphoglycerate, the pH dependence of all the key reactions (enzymes) needed to be incorporated into the expressions for the various equilibrium and kinetic constants.
An additional uniform activator, S, is incorporated into the gene expression function using the same thermodynamic formulation as described above: 7 where Here, S binds to DNA with an affinity KS and cooperatively binds with polymerase with a factor cSP.
The microscopic information of the electrode surface is incorporated into the response expression in the form of power spectrum of roughness.
Following confirmation of the entire nucleotide sequence, the fragment was then incorporated into the eukaryotic expression plasmid pcDNA3.1.
Similarly to the NLMS algorithm, performance improvements can be expected if the variable switching and mixing factors, (18) and (21), are incorporated into the correlation expressions.
Data were incorporated into the Hope Laboratory Expression Pattern Database [ 18]with details of the DNA fragment assayed, a text description of the GFP expression pattern, example images and further information about each strain generated.
Although all five unnatural amino acids were incorporated into GFP, the expression levels of GFP varied for the different unnatural amino acids, mostly likely due to differences in the efficiency of amino acid charging and/or amino acid uptake.
The effects on the oxidation rate of mass transfer, particle surface, oxygen partial pressure, and temperature have been considered and incorporated into the derived rate expression.
When wild type expression data was incorporated into the cluster analyses, gene expression profiles appear similar in wild type and tud progeny experiments, based on visual inspection (Additional file 6); only genes with expression in tud progeny were included in the cluster analyses.
