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Member checking entailed sending a summary of the sessions to all participants and inviting their comments, which were then incorporated in the transcripts.
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The shortest transcript (T6) initiates in exon 7, lacks exon 32 and 33, and terminates using an alternative exon, which is not incorporated in the other transcripts.
However, this exon was probably not symmetrical (see Fig. 2B) and, due the splice frame rule, only the ancestral or the duplicated exon could be incorporated in the coding transcript without disrupting the open reading frame, a classical case of mandatory alternative splicing [ 48, 49].
Our screening approach found 290 significant genes from the over 47,000 transcripts incorporated in the Affymetrix Human Genome U133 Plus 2.0 expression array.
In these 190 primary NSCLC samples, we correlated BORIS expression patterns within each tumor with expression of all transcripts incorporated in the U133 Plus 2.0 array by calculating a correlation coefficient using Excel.
Comparison of transcriptome and proteome data identified downregulated transcripts or proteins potentially incorporated in the oocyte by endocytosis.
These neglected UTR regions and novel genes increase APA extent and transcript diversity, which should be incorporated in the search of regulatory elements such as microRNA targets.
Two members of the research team double-analyzed a subset of the transcripts; disagreements and insights were discussed and alternative interpretations were incorporated in the analysis.
Such testimony shall be mechanically or stenographically recorded, and a transcript thereof shall be made and incorporated in the record of the investigation.
Biased amplification of the 3′ end of transcripts does not have a major impact on the overall transcript profile due to the 3′ bias of probe sets incorporated in the array design.
Instead, it is incorporated in the Cayman Islands.
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