Over the past two decades, advances in molecular biology have allowed molecular signatures to become increasingly obtainable [ 1] and incorporated into determining cancer prognosis and treatment [ 2].
It is also challenging to characterize the incorporated fluorophores due to the inaccuracies in determining their number, brightness and photostability from ensemble assays.
Inasmuch as tumor heterogeneity is one of the crucial factors in determining possible outcomes of anticancer treatment, it has to be incorporated and investigated in mathematical models.
We suggest that the φ angle distribution of ThT molecules incorporated in amyloid fibrils can differ from that in rigid isotropic solutions and this can be an important factor in determining the fluorescence quantum yield of bound ThT.
Protein was harvested and counts incorporated in macromolecules were determined.
The cellular DNA was collected on Whatman GFC filters using a harvester (Cell Harvester 8, Nunc), fixed with 96% ethanol, air dried and incorporated radioactivity was determined in the presence of 1 ml of scintillation solution (OptiPhos Hifase 3) in a liquid scintillation counter (Packard 1600 TR, Canberra Company).
Incorporated radioactivity was determined in counts per minute (CPM) using a scintillation counter (Beckman Coulter), and equal counts from each sample were mixed with glyoxal loading dye (Ambion).
A threshold at ∼0.80 Gy for incorporated plutonium was determined in a multifactorial analysis of lung cancer dose response relationships for 500 Mayak nuclear workers, 162 cases with cancer, and the control group of 338 workers who did not become ill with cancer in the same period.
The soil arching effect is incorporated in the study to determine the stresses acting on stone columns as well as soft soil.
Gene amplification was defined as a ratio greater than two, based on values determined by Vysis Inc and incorporated in their guidelines for using the PathVysion™ system to determine amplification levels of the HER2/neu gene in breast cancer in a diagnostic setting (Bartlett et al, 2001).
