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The fragment of CD9P-1 gene was amplified using the forward primer (5′-CGG GTCGAC AGGTCCACTGCAGGGGGTTA-3′) in which a SalI site was incorporated (italic letters) and the reverse primer (5′-CGC ACGCGT TTCCCCTTTGGAAGAGAGAGCA-3′) in which a MluI site (italic letters) was incorporated, generating a 392 pb fragment.
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Each signal peak is proportional to the number of nucleotides incorporated (e.g., a triple dTTP nucleotide incorporation generates a triple higher peak).
A variety of organic and inorganic pigment mixtures could be incorporated, generating translucent and opaque coatings, respectively.
In this technique signal synthesis [4] and signal averaging processes are incorporated to generate a desired impulsive signal from acoustic driver(s) and to eliminate background noise (flow noise) from in-duct and far field signals, respectively.
Petrophysical properties of the reservoir obtained from well log data and CCA, SCAL experiments of the 64 plugs, were incorporated to generate a single well model.
In the forward primer (5'- AAGACTAGCGAAGAGTGaTT-3'), a mismatch nucleotide, the lower case a, was incorporated to generate a Hinf I restriction site when Col genomic DNA was used as a template in a PCR reaction with the reverse primer (5'-TTCGACACATGAAACTCACC-3').
The viral protein was eventually adopted by its host and incorporated into the fungal genome, generating a family of proteins that are now critical to producing spores, invading host tissues and other fungal characteristics.
In the reverse primer, four mismatches, shown in lower case, were incorporated to generate an Nco I recognition site.
Use of an excess of PCR cycles (exhaustive conditions) ensures that the tag primers become fully incorporated into PCR product to generate a similar yield of amplicons between independent assays.
Their voices must be heard and incorporated in political, economic, and social arenas to generate a sustainable peace.
We previously generated a permanently-transfected pituitary cell line (α−23 cells) which incorporated a human TSHα promoter/luciferase cDNA construct (Figure 1) that was negatively-regulated by T3 [23].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com