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In brief, residual trait values were obtained from a mixed model that incorporated fixed environmental effects as well as the random effect of breed (herd of origin) and sire, thereby accounting for possible stratification in the data, using ASReml [ 38].
The mixed model applied to the data incorporated fixed effects for known covariates (age, age2 and sex), and variant components were calculated for genetic and residual environmental effects.
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In their analyses, they pooled the data from the 13 countries but incorporated country fixed effects in their models, along with variables representing a range of respondent characteristics.
Dummies incorporated in fixed bridges were included in all analyses but were not counted as present permanent teeth.
Yahoo! was not incorporated to fix China".
After incubation at 28.5°C for 30 min, BrdU-incorporated embryos were fixed with 4% Paraformaldehyde (PFA).
System-level characteristics and strategies were incorporated as second-level fixed effects in patient-level regressions.
The cells were fixed and incorporated BrdU localised using anti-BrdU (Bu20a; Sigma, Dorset, UK) and a standard Avidin-Biotin detection system.
BrdU (50 μL of 10 mM stock) was directly applied to HH24 embryos in ovo; 4 hr later, embryos were fixed, and incorporated BrdU was detected as previously described (Wendler et al. 2003).
Random effects were incorporated for provider- and site-level characteristics, and fixed effects were incorporated for patient characteristics that significantly varied between groups.
When municipality fixed effects are incorporated, it can be seen in column (4) that Seguro Popular reduced the likelihood of receiving private transfers by 3.58 % points, where this coefficient is significant at the 5.0 % level.
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