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The linkage map also incorporated EST-SSR markers; details on the use of this data have been reported previously [ 31].
We also incorporated EST-based SNPs, available through existing 5'-nuclease assays, into our genetic map because the substantial majority of RAD tags reside in non-coding sequences.
The information also includes names of ESTs incorporated into each 14,962 EST cluster and tables with the association links between EST accession numbers and EST clones.
In a previous publication [11], we incorporated Expressed Sequence Tags (ESTs) together with complete protein sequences plus a morphology matrix into a phylogenetic analysis of the seed plants.
Our algorithm measured the robustness of evidence provided by the spliced EST annotations and incorporated evidence from annotations of UCSC Known Genes and GenBank mRNA.
GarlicESTdb is the first incorporated online information database of EST sequences isolated from garlic that can be freely accessed and downloaded.
More U12-type introns, particularly those with AT-AC termini, were reported in U12DB because the database incorporated introns predicted by mapping EST data in addition to genomewide annotation.
A custom cDNA microarray was utilised which incorporated blackcurrant Expressed Sequence Tags (ESTs) derived from buds at various stages of dormancy.
In summary, the GarlicESTdb is the first incorporated online information database of garlic EST sequences that can be freely accessed and downloaded.
However, genomic data (genes and pseudogenes), coding sequences (cDNA or mRNA) and expression data (EST) will be soon incorporated, in order to help exploring some crucial issues: how many LEAP are encoded in each particular organism?
The review focuses on healthy muscles and incorporated only a handful of studies that used EST or functional electrical stimulation (FES) of impaired muscles.
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