Sentence examples for incorporated during the second from inspiring English sources

cDNA was synthesized during the first cycle and biotin-labeled nucleotides were incorporated during the second in vitro transcription reaction.

The biotin-labelled nucleotides were incorporated during the second step in vitro transcription reaction by using the gene chip IVT Labelling Kit (Affymetrix).

The land was prepared by plowing and harrowing, and recommended doses of fertilizers (40 : 40 : 40 : 2 N P K Znkg ha−1) were incorporated during the second harrowing prior to field levelling.

BrdU, which is the analog of the DNA precursor thymidine, is incorporated during the first major round of cell cycle activity.

The normalized cDNA was then amplified using primers complementary to the adaptors incorporated during the second-strand reaction.

Adaptors incorporated during the first strand synthesis were partially removed by SfiI digestion (8 U/μg of cDNA).

27 The TRIS ligands were pre-functionalized before being introduced into the reaction mixture, that is, the organic ligand is formed first then incorporated during the constitution of the hybrid cluster.

The second primer is an M13 primer that acts as a forward primer once the M13_polyC is incorporated during the initial PCR cycles.

Relevant feedbacks were incorporated into the second reconciled Malay version.

The spacer and linker moieties for conjugation are incorporated in the first step during formation of the 7-membered diazepine ring.

In this method, dUTPs are incorporated only into the second strand of cDNAs during cDNA synthesis and these dUTPs are then excised prior to library amplification, ensuring that only the first cDNA strand is efficiently amplified.