The studies described herein were designed to determine whether doxorubicin (DOX) pharmacokinetics (PKs) could be described by a physiologically based PK model that incorporated macromolecule‐specific binding and organ‐specific metabolism and excretion.
When different amounts of methylated DNA were incorporated, the binding activity was reduced proportionately in vitro (Figure 7A).
Second, extensive secondary structure in the mRNA, such as the presence of RNA-associated proteins and the specific subcellular localisation of the target mRNA that render the transcript inaccessible to siRNA-incorporated RISC binding (Holen et al, 2002).
Bar-Joseph et al. [36] incorporated DNA-protein binding results along with expression profiles in order to describe a genome-wide regulatory network.
More broadly, this tile-based approach involves "static" monomers incorporated by simple binding to the growing structure without explicitly designed internal state changes.
The model presented shows that receptors captured to the synapse following a diffusion/trap model are first incorporated at peripheral binding sites within the PSD, assuming uniformly distributed anchor molecules.
Protein purity was c. 70%, based on Coomasie staining of SDS-PAGE (see Additional 1: Figure S2A); although limited degradation products were present, only full-length protein was incorporated into DNA binding histone tetramers (Additional 1: Figure S2B).
We incorporated genome-wide binding data for TFs (Oct4, Sox2, Nanog, Stat3, Smad1, cMyc, nMyc, Zfx, E2f1) and other regulators (Suz12) implicated in the maintenance of pluripotency and self-renewal, which were obtained by chromatin immunoprecipitation (ChIP) and massive parallel sequencing (ChIP-seq) by Chen et al (2008) [ 16].
We incorporated BMP-2-binding heparin microparticles (HMPs) into a hydrogel scaffold to improve spatiotemporal control of BMP-2 delivery to large bone defects.
