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This could be explained if the Q169 mutants have reduced affinity for the nucleotide that would be incorporated at this position.
Interestingly, the shR-P16 construct showed little mutant-specific discrimination, knocking down both wild-type and mutant targets with high efficiency (20% and 10% remaining of the wild-type and mutant targets), and indicating high tolerance of the single nucleotide mismatch incorporated at this predicted shRNA guide strand position.
A linear model is incorporated at this stage to eliminate positional effects.
Presumably these mitochondrial specific proteins MRPL39, MRPL44 and MRPL45, which have been incorporated at this exit site [20], have evolved to facilitate accurate co-translational insertion of the nascent peptide into the IMM.
Ser and Thr substitutions at V13′ also result in hypersensitivity in a variety of nACh receptor subtypes and it therefore seems that polar residues can be incorporated at this location in the pore with comparable effects (Briggs et al., 1999; Dash and Lukas, 2012).
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This sequence was incorporated at the 3'- PCR primer sequence in the respective order (Fig. 1C and Fig. S6).
Analysis of this sample showed that no deuterium was incorporated at the α-position; hence, the H-labeled product 4 b must have resulted from enzymatic water addition.
In our country this new system was employed in Remington rifles incorporated at the beginning of the 1870 decade.
As new technologies like this develop we aim to consider where safeguards can be incorporated at the drawing board stage".
AstraZeneca's co-operative is incorporated at the group's Dutch offices, eight miles east of The Hague.
Wheat bran as BSD-treatment was incorporated at the rate of 1 kg/m2 using a rotary tiller.
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