This technique can be useful in studying complex protein interactions, since theoretically, the UAA photo-cross-linker can be site-specifically incorporated at any site of a protein.
As shown in Figure 3B, both 566FF-zipper fibers were incorporated into mature virions; the 566FF-R-R34 fiber was incorporated fully (Figure 3B, lane 3) while the 566FF-EE12RR345L was incorporated at a reduced level.
The increase of galactose groups incorporated at a fixed content of Ph groups improved mechanical properties of the resultant gels.
Iron was successfully incorporated at a value of 0.28 at% on the surface of the catalyst structure.
In addition, a single photoreactive probe incorporated at a unique site in actin nascent chains of different lengths reacted covalently with multiple TRiC subunits, thereby indicating that the nascent chain samples the polypeptide binding sites of different subunits.
Third, the genetically engineered streptavidin into which a redox-active non-natural amino acid, anthraquinonylalanine was incorporated at a specific site was synthesized and characterized as an artificial redox protein.
This study investigated the effects of pea (Pisum sativum L). particle size incorporated at a level of 227 g/kg in two diets differing in their nitrogen content using a 2 × 2 experimental design.
The folding pathway of the third domain of PDZ from the synaptic protein PSD-95 was characterized using kinetic and equilibrium methods by monitoring the fluorescence signal from a Trp residue that is incorporated at a near-surface position.
Approximate traffic equilibrium conditions can be integrated within the parsimonious traffic models to develop regional routing strategies, while detailed route choice strategies can be incorporated at a later stage in a hierarchical framework.
WPC was replaced with 70 vol% expanded vermiculite (EVM), NMK were incorporated at a rate of 2, 4, 6, 8 and 10% as partial replacement by weight of EVM.
