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In order to do this, we must find an efficient manner to incorporate a sequence based cost in addition to the reconciliation cost of Doyon et al.
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As the model is multi-frame-based, the degradation process incorporates a sequence of composite operators— W 1 B 1 D 1,…,W k B k D k,…,W M B M D M —which are applied to u to produce y 1,…,y k,…,y M, respectively.
Second, the presence of the capture oligonucleotides on the gold nanoparticles can be used to easily conjugate other targeting moieties which incorporate a complementary sequence.
We found that those libraries which incorporate a linker sequence (experiment 2) and utilize more input template in their preparation display a much greater bias for reads towards the 5′ ends of genes.
The results were incorporated into a sequence database that allowed stand-alone BLAST searches to be conducted.
These previous studies indicated that the DUB/USP17 proteases are an unusual family of genes incorporated into a sequence which has tandemly replicated in at least three separate species.
A straightforward extension to incorporate a known sequencing error rate was proposed by Felsenstein (2004).
In order to incorporate a newly sequenced genome in our analysis, it is necessary to (1) predict genes and (2) assign COG categories.
This adaptor incorporated a kozak sequence (GCCACCATGG) and a consensus excretion signal sequence (MGWSCIIFFLVATATGVHS).
Additional error reduction strategies will depend on the residual error rate present, which can be indicated by a separately barcoded monoclonal CDR3 control incorporated into a sequencing reaction.
To multiplex sequencing of different Bar-seq libraries, we developed a PCR-based method for library preparation that incorporates a unique sequence index for each library (see Materials and Methods).
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