Some inconclusive sequences were found in the three genomes, due to frequent high homology between some neisserial genes (opa for instance) and duplication of some regions (mostly IS), and were further tested by PCR and sequenced.
The study demonstrated the importance of validation studies to better understand the system(s) used, to highlight potential limitations in specific target regions, and to identify robust and/or inconclusive sequences to refine diagnostic interpretations.
BLAST results against the Tetraodon nigriviridis genome were inconclusive, as most sequence contigs matched to "un_random" sequences (sequence contigs and scaffolds that have not been mapped to any Tetraodon chromosome) that collectively spanned over 130 Mb.
When the indel report was inconclusive, the underlying sequence traces and the consensus sequence were analyzed using Cloe, the TIGR sequence editor program, to correct scoring of the indel.
Evidence from FoxP2 expression studies in avian brains have proven inconclusive [9], and sequence comparisons between non-vocal learners and song-learning birds as well as a small number of vocal learning mammals, have not identified specific mutations related to this ability [4], [9], [11].
Control samples for which test results were inconclusive were subsequently sequenced.
Of the 257 sequences, 10 sequences showed inconclusive or no matches.
iPCR analysis led to successful identification of IMGs in 21 of the lines while for the remaining 20 lines iPCR results were inconclusive, yielding either repetitive sequences that have multiple matches in the zebrafish genome, sequences that mapped to short contigs devoid of protein coding genes, or sequences that did not map onto current assembly of the zebrafish genome.
While previously, newborns and infants often underwent invasive, time-consuming, and costly diagnostic procedures yet in some cases with ambiguous and inconclusive results [11] next-generation sequencing approaches are increasingly implemented in daily clinical practice.
Comparison of Kupe virus L segment sequences was inconclusive in determining its relationship to DUGV.
