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Using mesoporous materials for molecular imprinting is an effective approach to overcome this barrier and to remove the limitations of the traditional molecularly imprinted polymers which include incomplete template removal, small binding capacity, slow mass transfer, and irregular materials shape.
The most common mechanism is incomplete template extension, when a partially extended sequence from one sequence reanneals to another parent in the next cycle of PCR.
MIP films prepared by free-radical polymerization with acrylic or vinylic monomers sometimes suffer from incomplete template removal during MIP preparation.
This apparent incomplete template utilization was not due to inefficient probe hybridization (see Figure 4 and 'Discussion'), but, instead, was likely limited by the accessibility of the DNA template due to surface interference.
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In contrast, all methods based on random priming can reinitiate amplification from incomplete templates, thereby providing a more complete genomic coverage.
Most non-LTR families usually consist of a large majority of 5' truncated copies, which has been attributed to incomplete reverse transcription, template switching, or other mechanisms [ 32- 35].
TAL is a modular declarative language that supports the specification of templates: incomplete hypermedia compositions.
Also the incomplete extension of a template due to insufficient nucleotides within a flow can cause a read to get out-of-sync.
Even in instances of incomplete liftoff, soaking the template in concentrated nitric acid for 10 min generally sufficed to dissolve any residual metal, making the electrode ready for the subsequent growth of new nanowires.
The high background is likely due to non-specific hybridization of incomplete reverse transcription, or template-independent, products produced during the amplification steps that bind with less specificity than their full-length counterparts [ 31, 40].
In fact, Dodd-Frank is too idiosyncratically American and too incomplete to be a true template for others.
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