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The results implied that incomplete sequencing of the genome may cause the omission of genomic regions containing the SelL gene.
The variations in fragment size are also partly due to incomplete sequencing of the flanking sequences of SLA and the putative protein-coding gene CMQ_5208.
Reasons for the incomplete sequencing of the remaining strains include low concentrations of RNA and overlapping peaks in portions of the chromatograms.
In addition, the WES technology is also subjected to other limitations, such as the lack of a (small) proportion of genes/exons on the enrichment platform as well as the inefficient capturing and/or incomplete sequencing of some regions.
NA indicates no amplification (failures), likely because of mutations in the priming sites or incomplete sequencing of VNTR regions and does not necessarily indicate absence of a VNTR region.
Though no SECIS information is available due to the incomplete sequencing of this gene, homology analysis shown in Figure 1 provides enough evidence to classify it into this novel family.
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First, after removing the incomplete sequences of the horse and Hottentot golden mole, we used the maximum likelihood method [62] implemented in the PAML package.
The incomplete sequence of BoCCD4-5, suggests a more distant relationship to the BoCCD4 small family defined by the previous proteins.
The incomplete sequences of those ABC genes might be because the genome of silkworm is not at completion and annotation of the entire genome is not complete.
Since the genomic region of the PITX1 was not available due to partially incomplete sequences of the three-spined stickleback genome, the sequence of this gene (GenBank: AY517634.1) was used.
Other contigs contain incomplete sequences of BmCR1B retroelement to a greater extent, either due to their position at a contig border (Ctg034636; Ctg022133; Ctg011753; Ctg028411; Ctg016714) or to insertion of sequences of an unknown origin (Ctg010781 and Ctg004626).
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