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In this study, we have cloned and sequenced a 24-kb fragment of a DNA locus upstream of the previously reported but incomplete putative ORF9 of L. lactamgenus.
A homologous, but incomplete putative N-terminal extension was observed for Sm2CPB.2 (Fig. 5A).
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The sequences marked "*" are incomplete, or from putative pseudogenes, and their mature polypeptides are not predicted.
If there was incomplete coverage, the putative transcript was improved using the JGI gene models and the Blast results.
Prophage 1 is a putative incomplete prophage and has a genomic length of 13.6 kb and G+C content of 32.9%.
A fourth homolog might be present in the genome as well, indicated by the putative incomplete CgNHR40 NR (Additional file 2), the sequence of which could not be fully resolved.
Sequences described as partial, incomplete, fragment, predicted, putative and hypothetical as well as duplicates and isoforms were excluded resulting in a total of 299 sequences from 51 species analyzed.
This software allowed us to improve on our first manual sequence analysis performed in a subset of zebrafish genes with homology to HC21 human loci (data not shown), and to quickly obtain a useful list of putative incomplete ORFs in zebrafish.
In B. floridae, due to incomplete genome sequencing, the putative Yorkie ortholog we obtained is very short, 86 aa in length and unlikely representing its true sequence.
Sequence contigs with lack of start and stop codon due to incomplete sequence coverage or putative sequence errors causing frame shift mutations were not included in the analysis.
Finally, these investigations were not able to identify putative pseudogenes, incomplete hemoglobin genes or footprints of historical hemoglobin genes within the hemoglobin gene clusters or elsewhere in the genome.
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CEO of Professional Science Editing for Scientists @ prosciediting.com