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However, specific activities of purified hydrogenases from these systems are significantly lower than activities of hydrogenases isolated from their native hosts, likely because of incomplete enzyme maturation [22], [24], [25].
These other tags may also arise because of alternative splicing or incomplete enzyme digestion.
Other tags may also be generated because of incomplete enzyme digestion in practice.
The possibility of genotyping errors because of incomplete enzyme digestion, leading to inflated heterozygote scores (this would not affect Pan IAA homozygotes) was thus examined.
Other obvious factors resulting in unassigned tags, such as experimental artifacts (incomplete enzyme digestions and ligations, as well as inefficient cloning procedures), are not discussed here in details.
Also, understanding the relative advantages and limitations of the various enzyme DNA hydrolysis cocktail protocols is critically important because of the potential difficulty in observing certain types of adducts resulting from incomplete enzyme hydrolysis.
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Until effective gene-replacement therapy is developed, expensive, and at best incomplete, enzyme-replacement therapy is the only hope for sufferers of rare lysosomal storage diseases.
Since Ras-like G proteins are considered incomplete enzymes, they require the presence of GAP to form a GDP aluminium fluoride (AlFx) complex, which mimics the transition state of phosphoryl transfer (Mittal et al, 1996; Daumke et al, 2004; Gremer et al, 2008; Veltel et al, 2008a).
A faint band in one of the AS samples may suggest a similar copy number variant or the possibility of incomplete restriction enzyme digestion.
The high frequency of the mutation, together with the incomplete effect of enzyme inhibitors in adults, supports a contribution of this kdr mutation in deltamethrin resistance.
The non-β-oxidative pathway remains incomplete and the enzymes resulting in the formation of benzaldehyde are still unknown.
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