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Damaged or incompatible ends require remodeling prior to ligation, tasks accomplished by a variety of processing enzymes, notably DNA polymerases μ and λ, PNKP (polynucleotide kinase and phosphatase) and Artemis.
It is known that DSB repair of incompatible ends always involve deletion of a few nucleotides (Guirouilh-Barbat et al. 2004; Nick McElhinny et al. 2005; Lloyd et al. 2012).
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LigIV can ligate incompatible DNA ends, across gaps at DNA ends and poly-T single strands [106,107]; this, with the exception of poly-T single strands, is stimulated by Ku and XLF [106,108,109].
In this regard, the observed activation of DNA-PKcs may also reflect that incompatible DNA ends are generated.
I suspect it is because they put so much stress on finding a person who is religiously the same as they are -- and then dozens of far less explored, and incompatible variables end up driving them crazy -- and causing them to leave each other.
One of the main reasons that security and liberty remain, in the end, incompatible for Machiavelli and that the latter is to be preferred may surely be traced to the "rhetorical" character of his republicanism.
This suggestion is not meant to be defeatist, but rather to highlight our pursuit of incompatible policies to absurd ends without recognizing the incongruities.
He added: "Sometimes inconsistents seemed to possess them at once in joint sway; but they never compromised: they pursued the logic of several incompatible opinions to absurd ends, without perceiving the incongruity".
And wouldn't I? Wasn't it more selfish to continue an incompatible partnership than to end it?
NHEJ is initiated by the alignment of broken, largely incompatible, and often damaged DNA ends using limited base pairing.
Polystyrene and polybutadiene are incompatible, so that the polystyrene end-groups associate together to form domains of glassy polystyrene in a sea of elastic polybutadiene.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com