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The results were not appreciably different than those for the more inclusive dataset.
For model development it is better to include only those sample points that are well documented than have a more inclusive dataset which includes gross geocoding errors.
The results of all the pairwise alignments were bundled together to form one inclusive dataset.
As a comparison, an inclusive dataset was also generated containing all samples from above, plus several more from liver.
The third most inclusive dataset includes the previous two datasets plus genes involved in cancer biochemical pathways and cancer associated biochemical functions (CancerGenes).
Significantly aligned, overlapping sub-sequences were merged and regions in other species that mapped to the same sub-sequence in N. vitripennis were identified to yield one inclusive dataset (see Methods).
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The third analysis, the phylogenetic incompatibility test only rejected the null hypothesis of random mating for the inclusive datasets of VGIII and VGIV (Table 5).
Analyses were also run using the all-inclusive dataset.
Analyses were also run using the all-inclusive dataset and the supertree compared to the re-grafted one to test the effects of constraining monophyly.
The main strength of this study was an inclusive, accurate dataset through strict and specific data collection criteria over a period of 8 years.
An inclusive, accurate dataset was used (from mandatorily collected hospital episode statistics (HES) data for Liverpool), ensuring the identification of almost all relevant data cases, therefore minimising the selection bias.
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