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Based on our previous findings, we selected 5 different anti-SMN sequences that demonstrated promising effects on upregulating exon 7 inclusion (Figure 1B) [1B].
U2AF65 knockdown also altered BIN1 splicing: unlike PUF60 depletion, which favored exon 12A skipping (Figure 6F), U2AF65 depletion promoted exon 12A inclusion (Figure 6F).
Finally, (5) although FUS/TLS showed no direct binding to the stem-loop IDX-rasISS1 (Figure 2 C, right), assays have indicated that FUS stimulated IDX inclusion (Figure 3 and Figure 4B).
In turn, 214 were deemed suitable for inclusion (figure 1).
A total of 705 exposed children never received IPT; there were 156 children from 'case houses with no inclusion' and 549 children from case houses with inclusion (figure 2).
Transient expression of this synthetic pre-mRNA yielded a splicing pattern comparable to the endogenous transcripts (∼10% inclusion, Figure 2B, lanes 1 and 2), indicating that most relevant regulatory elements are maintained in the minigene.
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In both veins, two-phase aqueous inclusions coexist with one-phase carbonic inclusions (Figure 2c).
Except for sample E4 (hosted by pillow lava), the quartz veins contain two types of inclusions: one-phase carbonic inclusions and two-phase aqueous inclusions (Figure 2c).
TEM study revealed the appearance of non-membrane-bounded cytoplasmatic electron-transparent inclusions (Figure 3b-f), which were absent in glucose based-cell cultures (Figure 3a).
The average F and Cl concentration in the melt inclusions is 156 ± 44 ppm and 399 ± 45 ppm, respectively, demonstrating that F and Cl concentrations are relatively low and clustered compared to other Pacific rim melt inclusions (Figure 2).
F and Cl concentrations in Iwate volcano are clustered (relative standard deviations of F and Cl are 28 and 11%, respectively) and lower than most of other arc melt inclusions (Figure 2).
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