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Multiple crystals were concomitantly soaked in the same vial for all guest inclusion experiments.
After guest inclusion experiments, crystals were manipulated in NVH immersion oil for selection and mounting.
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We then performed the inclusion experiment with chiral target 1 R--menthyl acetate (2), which was also used in our previous study with 1a to afford host guest complex 1a2 (Fig. 2a).
We generally recommend that crystals are soaked in neat target guest molecules for the inclusion experiment, which was also done by Buchwald and co-workers for a thioperoxide guest (Vinogradova et al., 2014 ▶).
For the guest inclusion experiment, the crystals were submerged in either neat trans-anethole, guaiazulene or (1 R --menthyl acetate at ambient tempeR --menthyl2 d in a small glass vial.
Additionally, the risk of introducing crystal imperfections during the inclusion experiment through heating was avoided, though it is possible to warm these crystals to 323 K in an analogous manner to the described protocol by Fujita and co-workers, if necessary (Inokuma et al., 2013 a ▶, 2014 ▶).
The dye-inclusion experiment: Fresh crystal samples of 2 (3 mg) and 3 (3 mg) were soaked in a solution of Rhodamine 6G (60 m m) in methanol for 12 h.
These include semi-automated capture of large volumes of data, identification of diurnally and nocturnally active PSC members, ready inclusion of experiments in field survey design, reduced impacts of unintentional human interference on survey results, and greatly reduced cost relative to direct observational studies.
Cells were serum-starved by culturing in serum-free medium for 24 h before inclusion in experiments.
The authors are requested to address (by inclusion of experiments or appropriate modification of the text) the following points in a revised manuscript: 1) Figure 1.
Regardless I don't think inclusion of experiments with non-isogenic lines is acceptable for a journal of eLife's standing.
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