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The inclusion body solution was then slowly pumped at 1 mL/min.
The inclusion body solution was injected into refolding buffer containing 100 mM Tris-HCl (pH 8.0), 2 mM EDTA, 400 mM L-arginine-HCl, 0.5 mM oxidized glutathione, 5 mM glutathione, 0.1 mM PMSF, and 0.1 mM NaN3.
To purify the HLA-A2 and HLA-G1, the inclusion body solution of β2m and peptide (TLACFVLAAV, derived from human SARS-CoV membrane protein for HLA-A*0201 HLA-A*0201HLQL, derived from cellulandendogenous pRIIPRHLQLstone for HLA-G1) deriveded in DMSO, were injected into the refroming buffer, and then the incellularbody solution of thendogenousain was later injected into the refolding buffer.
Direct culture supernatants were used for LipA, LipC and LipCmut immobilization, whereas LipI.3 was immobilized from a solubilised inclusion body solution, thus skipping the high costs and time-consuming steps of protein purification.
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Unfortunately, PoAbf62A formed inclusion bodies in solution because of its N-glycosylation sites.
The recombinant kurtoxin was expressed as an inclusion body, solubilized in a denaturing solution, and then refolded in a refolding solution.
Through urea denaturation, Ni-NTA purification, dilution renaturation in renaturation solution, dialysis in PBS solution, and ultrafiltration, the inclusion body was purified into recombinant LAH-HBc protein.
Each solubilized inclusion body chain was diluted to 5 mg/mL in guanidine solution.
The CHMO inclusion body and RNase A type XII-A (Sigma-Aldrich) were denatured and reduced in a solution containing 6 M guanidine hydrochloride (GdnHCl), 0.14 M DTT and 2 mM EDTA in 50 mM MOPS buffer, pH 8.0.
Contributions: Inclusion Body Myositis Assn.
This disease is called measles inclusion body encephalitis.
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