However, currently, preformed scaffolds for cell delivery have drawbacks including the difficulty to seed cells deep into the scaffold, and inability for injection in minimally-invasive surgeries.
Responses included the difficulty to reproduce a bug, the misunderstanding of root causes, the lack of information in the initial report, the increase of the bug priority, incomplete fixes, and code integration problems.
Screw-retained implant restoration has also some disadvantages including the difficulty to get a right positioning of the access-hole compatible with a suitable aesthetic appearance and the aesthetic result of the access-hole restoration [16].
The limitations of this study include the difficulty to adjust confounding factors from smoking.
The limitations include the difficulty to differentiate colonization from infection in sputum cultures of patients with HAP.
In Finland the pilot for a non-blind, patient-managed trial on hormone therapy (HT) revealed several obstacles to the main trial, including the difficulty to discontinue HT and a negative attitude among Finnish physicians towards the trial [ 5].
The reasons why this approach is less effective in inducing clinical benefits include the difficulty to provide enough stimulatory co-signals to exceed heavily overweighted inhibitory conditions in the tumor microenvironment.
The limitations of this study include the difficulty to ascertain the evidence whether the women started or quit smoking in Sweden or in their countries of origin as well as the difficulty to interpret the concept of acculturation since we lack data on numbers of years lived in Sweden and age of immigration.
Clear disadvantages of primary cells include the difficulty to obtain a sufficient number of cells, the diversity of cells within the cultures as compared to other cell lines, and the fact that the cells are not easy to transfect, thereby limiting the possibility to alter the expression of target genes (via loss- or gain-of-function mutations) to elucidate their role in OA.
The most significant barriers across both time periods related to the domain 'environmental context and resources', and included the difficulty to predict preterm birth and the complex administration process, particularly within the context of time pressures surrounding unexpected or rapidly progressing preterm labour.
