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The process does not rely on commercial (hemi-) cellulases but includes enzyme production through Hypocrea jecorina (formerly Trichoderma reesei) on the pre-treated feedstock as key unit operation.
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The prestige of microorganisms is due to their high metabolic versatility, which allows the inference about its potential for biotechnological applications, including enzyme production for industrial and environmental uses.
To achieve these goals, scientific advances in enzyme technologies, including enzyme production and performance, are required.
Biologically, these chemicals share an ability to bind to a cellular receptor known as the aryl hydrocarbon receptor, which regulates the expression of genes that influence multiple functions, including enzyme production and hormone regulation.
Promising bioactivities including enzyme inhibition, production of singlet oxygen, purine oxidation and photoactivation have been observed for cyclometalated IrIII complexes.
Proteins encoded by these genes include enzymes catalyzing the production of building blocks for oil assembly, enzymes involved in oil assembly, enzymes regulating metabolic carbon partitioning between oil, carbohydrate and secondary metabolite fractions, and transcription factors which orchestrate metabolism at a more general level.
They included enzyme activities and GAG assay.
Other mechanisms have also been proposed, including decreased endogenous enzyme production, reduced energy expenditure on intestinal cell turnover rate and shifting production of volatile fatty acids and absorption of energy-yielding monosaccharides in the proximal intestine (Adeola and Cowieson, 2011).
Energy demands of on-site enzyme production included in the 2011 design contribute to reducing the electricity co-product and associated emissions credit, which is also dependent on the GHG-intensity of regional electricity supply.
The benefits of transgenic plant technology for industrial enzyme production include replacement of chemical processes that cause environmental pollution.
Optimization of major physico-chemical parameters affecting enzyme production; including medium composition, pH, cultivation time and temperature revealed that maximum enzyme production was obtained in a self designed medium (pH 6.0) containing 1% Dextran 5000 Da, after 24 h culture incubation at 37 °C.
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