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In whole-cell recording experiments, 2 mM kynurenic acid and 1 µM gabazine (Park Ellisville) were included to block glutamate-receptor (AMPA and N-methyl-d-aspartate (NMDA))- and GABAA-receptor-mediated synaptic transmission, respectively.
Cs+ in the pipette solution was included to block K+-dependent membrane conductance.
Baf was included to block lysosomal degradation during both ND-induced autophagy and basal autophagy (FM) (Additional file 1).
Picrotoxin (50 µM) and tetrodotoxin (300 nM) were included to block GABAA receptor-mediated responses and action potential-driven excitatory/inhibitory postsynaptic events respectively.
In some experiments 20 μg ml−1 of DC-SIGN blocking antibody [R&D systems (clone 120507)] or 50 mM N-acetyl glucosamine was included to block receptor DC-SIGN.
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