Sentence examples for included restriction from inspiring English sources
Methods to analyze recovered plasmids included restriction digestion analysis, PCR analysis with gene specific primers, Sanger sequencing, and PacBio sequencing.
PCR primers included restriction enzyme sites (underlined) compatible with the targeting vector assembly.
They were designed to exclude a 662 base-pair nucleotide sequence and included restriction sites to fit a pHis1 parallel bacterial vector.
Such events included restriction of freedom and compulsory treatment.
Primers included restriction sites for EcoRI and NcoI for subsequent cloning into the binary vector pMAA-Red [ 33].
Primers included restriction sites KpnI at the 5' and XhoI at the 3' of the target sequences.
But it included restrictions on this freedom, among them a specific dollar limit on investments.
This included restrictions on bank lending and a temporary halt to appreciation of the yuan.
The crackdown has included restrictions on the ability of the popular tabloid Blic to print its normal press run.
The sanctions also included restrictions on air travel and oil sales to Yugoslavia, though both were eased even before Mr. Milosevic's fall.
The DHS funding bill included restrictions on where new border wall could be constructed.
