Bin on x-axis represent the percentage of the hit's length included in the alignment to the Trinity transcript.
Included in the alignment are channels from the eight major classes of K+-selective channels from a wide variety of species, displaying varied rectification, gating, and activation properties.
These latter were designed before knowing our results on enzyme diversity; thus, the sequences from OM60 clade were not included in the alignment used to construct the primers.
All sequenced codon positions were included in the alignment, for introns and exons in arsB; however, every site containing gaps or missing data was then eliminated from the dataset.
A human TTV sequence was included in the alignment as outgroup (accession no. AB041007).
Selected HIV-1 subtypes/CRFs of geographical importance were also included in the alignment.
However, sequence divergence of the mature miRNAs of precursor 515-1 and 515-2 were small enough to be included in the alignment.
Only sequences in the rebuilt set were used, neither the native starting sequence, nor the intermediate random sequences were included in the alignment.
All sequences which had matches greater than 200 score bits were included in the alignment, and redundant sequences from the two searches were discarded.
Stop codons and triplets that were affected by 1bp or 2bp deletions in a respective ortholog were also deleted in all other sequences included in the alignment.
The following proteins are included in the alignment.
