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Primers and amplification protocols are included in supplements.
Possible reasons why electronic searches fail are multiple: lack of relevant indexing terms, inconsistency by indexers, reports published as abstracts and/or included in supplements that are not routinely indexed by electronic databases [ 21, 22].
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Detailed description of the methods used is included in Supplement S1.
Further details are included in Supplement 1.
PCR primers were designed using LightScanner Primer Design software (Idaho Technology, Salt Lake City, UT, USA) and the primer sequences were included in supplement table (available online).
Iron supplementation will be administered in case of anemia for children not requiring nutritional supplements (otherwise, iron is already included in nutritional supplements).
If you are an athlete or bodybuilder taking nutritional supplements to improve your performance, though, creatine might be included in these supplements and should be cut out.
However, the use of B-vitamin supplements was low (7%) and folic acid was generally not included in these supplements in the Netherlands in the late 1980s.
Preliminary in vivo and in vitro interaction assays are now included in figure supplements.
The nutrients included in the supplements and doses differed across studies.
The majority of supplements used in these studies also included protein, but were distinct from the protein/amino acid studies in which multiple micro-nutrients were not included in the supplements.
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