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Negative controls of sterile deionised water and catalase solution were included in duplicate wells of each assay plate.
8-point calibrators were run in the first and last column of each plate and 3-level controls were included in duplicate.
Eight-point calibrators were run in the first and last column of each plate and 3-level quality controls were included in duplicate.
To control for DNA contamination in the reaction mix, control tubes lacking DNA templates (NTC) were included in duplicate with the relevant set of primers in each experiment.
Six-point 10-fold dilution standard curves were generated for 28S, IL-2, IL-4, IL-10, IFN-γ, TNF-α, IL-12p40 and IL-18 using plasmid DNA and included, in duplicate, on each run.
Similar controls were obtained from a Saanen goat and included in duplicate for testing goat sera.
Similar(43)
Each specimen was included in duplicates in the array.
First, these ten markers are unique genomic regions in the human genome (i.e. they are not included in segmentally duplicated regions, nor do they have closely related paralogous sequences in the genome, as determined by homology searches).
In the meta-analyses included in our review, duplicate data extraction was performed in 28% of all papers and 50% of the HuGE reviews.
Two of four reviewers (JH, RJMcM, UM, JM) carried out data extraction from included papers in duplicate (the data extraction form template is in appendix 3 on bmj.com).
We used broad search strategies of published literature and reviewed all citations and included studies in duplicate that resulted in good agreement and transparent reporting.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com