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A unigene was considered expressed in one library if at least one sequence derived form the specified library was included in the assembly of the considered unigene.
Contigs generated previously from partial sequencing of the P' sub-genome ANR-selected BAC [ 17] were also included in the assembly of this clone sequence.
Sequences of cox1, cob and rrnL amplified from each species with universal primers and sequenced by standard Sanger method were included in the bioinformatic assembly of reads to confirm the correctness of assignment of each mitochondrial sequence ("bait" sequences as in [ 47]).
However, we cannot exclude the possibility that some of these reads may actually correspond to regions not included in the original assembly of the available reference nuclear genome of the O. sativa subsp. japonica cv.
However, the high percentage of core gene families suggests that most of the genes are included in the current assembly of genome sequence.
Other parameters like the percentage of reads included in the assembly or the amount of sequences assembled indicate how much of the actual transcriptome is captured in the assembly.
In order to determine the percentage of reads included in each assembly we mapped the reads of each species against the respective contigs of each assembly.
Larger insertions of sequence from another location in the genome are likely to be aligned elsewhere and not included in local assembly, thus limiting detection of insertions as the size approaches read length.
Above a 400 bp average read length, the percentage of reads included in the assembly process decreased until at 1,000 bp only 25% of reads were assembled together.
The percentage of reads included in the assembly was 51.78%, which is slightly smaller than the highest value of the ABySS assemblies (55.93%).
Previously characterized repetitive sequences from soybean available at the time of assembly were included in prescreening during assembly (Marek et al. unpublished results) [ 39].
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