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Top hit sequences were downloaded and included in alignment analysis using CLUSTAL X 2.0 software (Larkin et al. 2007) and phylogenetic tree construction by MEGA 6.0 software (Tamura et al. 2013).
speltoides (S) and Ae. tauschii (D), were included in alignment and distance analysis.
This allowed reads spanning exon boundaries to be included in alignment results and be recorded in BAM format.
Spinach psbO sequence was retrieved as cDNA [GenBank:X05548.1] because of the lack of ESTs and included in alignment for comparison (Additional file 2).
Similar(56)
Proteins lacking a significant portion of the protease-like domain were not included in alignments.
Human basepairs in each functional genomic class included in human-chicken alignments (Hs-Gg), and of D. melanogaster basepairs included in alignments with D. virilis (Dm-Dv).
Alternate alleles at variant sites based on partial genotype information or common variants were included in alignments with the –v option.
NITR sequences described from medaka, zebrafish, Southern pufferfish, channel catfish, rainbow trout, Japanese flounder and Tetraodon (Additional file 3) were included in alignments [ 2- 4, 10- 14].
The top BLAST hits to annotated or predicted genes from Basidomycota and Ascomycota fungi were downloaded and included in alignments within the program Geneious version 5.5.6 (Drummond et al., 2012) for 83 consensus sequences.
The pre-ensembl Sus scrofa genome database (http://pre.ensembl.org/index.html; version Sscrofa8) was searched using the blat option for gag, pro/ pol and env, with best matching sequences being also included in alignments.
Only alignment positions with high Jalview quality score had been included in the alignment selection.
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