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EGFP expression was detected for monitoring the efficient and comparable transduction; β-actin was included for sample loading control.
A total of 343 women, aged 25 60 years, attending gynaecological polyclinics in DR Congo were included for sample enrolment.
26 The EDSS is the 'referent standard' measure of disability in clinical trials in MS. The EDSS is included for sample description only, not as an outcome of the intervention.
Only the hits to NCI-20 proteins and bovine trypsin were included in identification counts; when all data were included for sample 1B, a total of 79 human protein groups were detected, along with three decoys and trypsin, though spectral counts were far lower for proteins not found in the NCI20.
Values were not included for sample types (BU EPA and DHA and CE DHA) for which the model fitting did not converge because the data did not follow the asymptote model (this effect can occur when there is too much variability in data).
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Information on days after symptomatic onset were included for samples for which this data was available.
As many participants as technical limitations permitted (i.e., materials and costs) were included for sampling.
We included for sampling several particularly heavily trafficked areas (e.g., Columbus Circle and Times Square), as well as four small urban parks (referred to as "pocket parks").
Two technical replicates were included for each sample, and sample plates were run on the Mx3005P™ (Stratagene).
Serum controls were included for each sample to determine whether any individual serum sample was toxic to the cell culture used.
Realtime PCR was performed on an Eppendorf realplex mastercyler (Eppendorf, Hamburg, Germany) in triplicate for each sample, and a control reaction without enzyme was included for each sample.
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