The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry.
The measured endpoints included cell growth, morphological aspect, chlorophyll autofluorescence, oxidative stress, and membrane permeabilization.
The variables we optimized included cell density and duration of infection, presence or absence of 2% DMSO, and impact of spinoculation.
These responses included cell proliferation, oxidative stress biomarkers (ROS, MDA, GSH, 8-OH-dG), and alpha-smooth muscle actin (α-SMA) expression.
The induction conditions included cell density prior induction (OD600nm), post-induction temperature, IPTG concentration and post-induction time.
Assessments on day 3 included cell number, and the extent of fragmentation and asymmetry, and on day 5, the developmental stage.
The control plants included cell line control plants (cv Cavendish selections GN and/or Williams) from QUT in addition to tissue culture plants of Williams, GCTCV 218 and DPM25 supplied by Mission Beach Tissue Culture Nursery, Queensland.
We included cell lines carrying "benign" (R521C, L) and "malign" (R495QfsX527, P525L) FUS mutations and systematically compared them to three control iPSC lines from healthy volunteers and to isogenic lines (Table 1).
Moreover, the comparison of R vs Y included cell function transcripts, like Luminal-binding 5-like, endonuclease V and three transcripts probably related to phenolic compounds, cinnamoyl- reductase 2-like.
Guests left with a bag of goodies that included cell phones.
The cell responses investigated in this study included cell adhesion and cell proliferation.
