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Four facilities in Uganda included as using CD4 testing prior to initiation in selected patients were doing CD4 testing in all adults but not in all children.
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In the second analysis, the importance of time since initiation of the different agents was explored (variables were included as use vs no use of a specific agent in a specific time window (eg, 'actrapid day 0–30'humaloglog day 365.25–730.5' etc)).
Detailed instructions of use are included, as well as complete Javadocs.
Toxicities, side effects and current proscribed uses are included, but only as used in the United Kingdom.
Bartholdi et al. (2014) is not included as they use the same components as LSCI.
The breast cancer sections known to stain for positive FXYD-3 were included as negative (using PBS instead of the primary antibody) and positive controls in all runs.
Sham transfections were included as negative controls, using transfection reagent only (−siRNA).
Items included as resource use were identified from previous economic evaluations 9, 18, 19 and key opinion leader interviews.
Nasal sprays, eyedrops, eardrops, local treatment in the mouth, inhalation corticosteroids, intestinal foams and suppositories were included as local use.
Also, some articles included as systematic reviews used relatively vague methods.
The same definitions for the hypertension, hypercholesterolemia and overweight for the background population and included AS patients were used.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com